Disinfectants Used Within Microbiology Laboratory

Disinfectants Used Within Microbiology Laboratory The purpose for this literature review was to examine the literature currently available to the general public on the application of a range of disinfectants used within a microbiology laboratory in both the public e.g. hospital laboratories and the private sector e.g. university laboratories. This literature review was carried out on the effectiveness of disinfectants so that previous and current knowledge on the use of these disinfectants can be analysed. This will help give an insight into the subject area and help with the preparation and production of the final report based on the research being carried during the literature review and research project. The main purpose of the research project being conducted was to compare the effectiveness of a variety of disinfectants especially Trigene which has been endorsed for use in the microbiology laboratories of the Greater Glasgow and Clyde health board and that of Virkon which is currently used by the microbiology laboratory at the University of the West of Scotland. The literature examined showed that the disinfectants used in clinical laboratories and any other area where microorganisms can cause a problem with cross-contamination, should be evaluated for their effectiveness against the range of organisms which might be encountered. It is an important requirement that the disinfectants being used are able to inhibit or kill the microorganisms quickly and by using the lowest concentration available. (Isenberg, 1985) A study conducted by Kasthjerg et al (2010) which looked at the effects of a range of disinfectants on the expression of virulence genes present in the bacterium Listeria monocytogenes. This study showed that effects on the virulence gene in the bacterium could be linked to the chemicals found in the disinfectant with some causing an inhibition of the gene while others showed an induction of the gene Disinfectants A disinfectant is a chemical which is widely used to eradicate a variety of microorganisms that are currently found in the samples received into a laboratory or are currently used within an educational setting for the teaching of microbiology to students. Disinfectants can have any of the following chemicals as their main active ingredient: halogenated tertiary amines, chlorine containing compounds, phenols, quaternary ammonium compounds and peroxygens. (Tyski et al, 2009) Thus the disinfectants can be divided into groups relating to the chemical present as the active ingredient and these groups will be discussed later in the report. It is important that certain criteria are met when classifying a chemical as a disinfectant, these include:- That the chemical components of the disinfectant will not have an adverse effect on the health of the user and if any health issue is realised then appropriate action can be taken to remove this risk i.e. use PPE such as gloves or respiratory mask. (Severs Lamontagne, 2002) It. is also important that the disinfectant has the ability to render inactive or kill a wide range of microorganisms including viruses, bacteria and fungi. (Severs Lamontagne, 2002) It is also important that a disinfectant does not have an adverse effect when used on equipment. The research conducted by Kim, Ryu Beuchat, (2007) showed that the effectiveness of certain disinfectants can be diminished when they are in contact with biofilm surfaces. It is currently thought that there is very little resistance to the disinfectants used due to the presence of a variety of chemicals considered to have an antimicrobial effect in each disinfectant. (Kasthjerg et al, 2010) Efficiency testing of disinfectants has been around for roughly 50 60 years ago. (Werner, 2007) In more recent years a working group was set up which set Standards which govern the microbial activity testing of chemicals used as antiseptics and disinfectants. (Werner, 2007: Tyski, S., et al, 2009) The introduction of these standards has allowed the efficiency tests to become more quantitative and produce lower margin of error. (Werner, 2007) Peroxygen This group of disinfectants consists of chemical disinfectants which contain the chemical peroxygen and includes Virkon which is one of the disinfectants which will be evaluated during the research project being carried out. Virkon is available as a powder and is diluted for use by dissolving it in distilled water to give the required concentration. Virkons method of action is through the combination of the chemicals chlorine, hydrogen peroxide and paraoxymonosulphate. (Alegente, G., et al, n.d.) These chemicals act by oxidation of the plasma membranes of microbial organelles. The effects of peroxygen disinfectants was investigated by a study conducted by Quilez et al, (2005) to discover what concentration of the disinfectant and time required for the disinfectant to be most effective when dealing with Cryptosporidium parvum oocysts. The literature review conducted by Severs Lamontagne, (2002) indicated that there was only a moderate risk of irritation to the user when the Virkon is in powder form. The Virkon may induce coughing, chocking or wheezing if inhaled while preparing the diluted disinfectant. However, when the powder has been diluted into a liquid form it is thought to cause no harm to the users skin or eyes. (Severs Lamontagne, 2002) As the suggested research to be conducted is related to the efficiency of disinfectants then it was important to review current literature which gives an indication of the effectiveness on a range of microorganisms. From the literature it was shown that Virkon is effective when used as indicated against a wide range of microorganisms including Gram positive and gram negative bacteria, viruses, fungi and spore forming bacteria. The study conducted by (Severs Lamontagne 2002) showed that these organisms were inactivated using a 1% solution of Virkon. A study conducted by Gaparini et al, (1992) showed that Virkon was effective when used against spores which had been cultivated onto nutrient agar. This study also indicated that the effects of Virkon were quick acting; this could be attributed to the high concentration coefficient of the disinfectant. Group 2 Halogenated Tertiary Amine This type of disinfectant is considered broad spectrum and biodegradable and is generally found in combination with detergents. (Health and Safety Executive, 2005). The halogenated Tertiary Amine group of disinfectants includes Trigene on which part of the research project is based. Trigene is available as a concentrated liquid or as a manufacturer diluted liquid. (Trigene Company) Trigene is also available in sachets which have been approved for use on submarines used by the Armed forces in the UK. (Severs Lamontagne, 2002) The findings of the study by Severs Lamontagne, (2002) showed that as with Virkon the use of Trigene was effective at rendering inactive viruses, bacteria and fungi when used in accordance with the manufacturers instructions. Health and safety tests were carried out on Trigene and the results have shown that the disinfectant poses a low risk to the users if accidently inhaled or ingested and there are no known health hazards with the use of Trigene. (MediChem international) Quaternary ammonium compounds This group of disinfectants is considered to be effective against most bacteria strains; however they are ineffective against viruses, bacteria which form spores and Mycobacterium. It is likely that this would not be an efficient disinfectant to be used within a clinical or university microbiology laboratory due to the risk of receiving samples containing spore forming bacteria, Mycobacterium species. The disinfectant can become inactivated by a low PH or the presence of organic material. (Health and Safety Executive, 2005) The mode of action for members of this group of disinfectants is that they damage the cell membrane which will result in cell leakage. (Ioannou et al, 2007) This study also showed that an increase in temperature increased the time taken to eradicate the bacterium. The study done by, Kim, Ryu Beuchat, (2007) showed that the efficiency of the disinfectant could be linked to the chemical structure of the Benzylalkonmium chlorides hydrophobic alkyl chain. It also showed that the presence of organic material (infant formula) can decrease the efficiency of the disinfectant. Hypochlorite and other chlorine-releasing agents This is a disinfectant which contains chlorine as its active ingredient, and an example of this disinfectant is Milton which is currently used to sterilise baby utensils. It is considered a broad spectrum disinfectant as it can inactivate or kill a range of bacteria and viruses. However, it is considered less effective against bacterial spores. As with the quaternary ammonium compounds this group of disinfectants has been shown to be inactivated by organic material and has to be prepared daily, this is due to the instability of the chemicals caused by concentration and temperatures at which the disinfectant is stored. (Health and Safety Executive, 2005) The hypochlorite disinfectant is provided in the form of Sodium Hypochlorite and it is commonly used within a clinical laboratory especially for the cleaning of equipment when recommended by the manufacture. Phenolic This group includes the disinfectant Hibetine which is currently used at the University of Glasgow and the active chemical is phenol. It has been shown to be a broad spectrum disinfectant which is effective against certain bacteria including Mycobacterium tuberculosis and some viruses, although shows no action against bacterial spores. The disinfectant is used with a combination of the phenolic chemical and a detergent. The advantages of using this type of disinfectant is that it is considered stable in undiluted form and organic material does not appear to have the same negative effect on the efficiency of the disinfectant as has been observed in other disinfectants.. Microorganisms an introduction The main purpose of conducting the research project was to observe the efficiency of disinfectants when used against a range of microorganisms. The microorganisms to be investigated include a Gram positive bacteria such as Staphylococcus aureus, a Gram negative bacteria such as Escherichia coli, a spore forming bacteria such as Bacillus cereus and a yeast e.g. Saccharomyces cerevisiae. The study conducted by Tyski, S., et al, (2009) used microorganisms which could easily be isolated from hospitals. The study carried out by Ioannou et al, (2007) suggests that a common feature of bacteria especially staphylococcus aureus can acquire a plasmid which contains genes which will allow the bacteria to become resistant to certain antimicrobials. The literature search carried out showed that the organisms which are commonly used in research to show the efficiency of disinfectants are commonly isolated from hospital patient samples. (Gasparini, et al, 1992) Gram negative bacteria Gram positive bacteria Yeast Sporing bacteria The use of disinfectants in combating the spread of infections from bacterial spores from organisms such as C. parvum, Bacillus anthraxis, Clostridium difficile and bacillus cereus is important as these are the most difficult of all microorganisms to eradicate. (Quilez et al, 2005) Discussion The literature reviewed has shown that disinfectants are an important part of the working environment of a laboratory as it is essential that the disinfectant has the ability to inactivate or destroy a wide range of microorganisms. The study by (Severs Lamontagne, 2002) showed that both Virkon and Trigene could be classed as efficient and broad spectrum disinfectants as they both killed a variety of microorganisms ranging from viruses (both enveloped and non-enveloped) to fungi and bacteria including spore forming species. The study by Kasthjerg et al, (2010) deduced that the disinfectants which contained the peroxygen and chloride compounds decreased the virulence gene expression; however, the disinfectants containing the quaternary ammonium compounds encouraged the expression of the virulence gene. Conclusion The study conducted by Severs and Lamontagne, (2002) suggested that Both Virkon and Trigene were effective at eliminating the bacteria which could poses a health risk to divers but they felt that as the Virkon product had managed to eradicate more bacterial and viral strains than Trigene and that these effects were produced quicker with Virkon so the conclusion was that this product (Virkon) should be considered a better disinfectant and be recommended for use by divers.

IPCSL Case Study Essay

In 1944, Debi Prasad Aggarawal had realized the need for a cold storage facility. Consequently he established a facility with the latest cold storage equipments from the UK which was mainly utilized by the potatoes harvest. Debi’s son, Jadish, succeeded him as Managing director in early 1960. Jagdish had successfully opened new markets and brought new clients when he started promoting the facility to fruits producers. In 1977, Sanjay Aggarawal, Debi’s grandson, joined the company with the task of supervising and the construction of the new 2500 MT facility in Azadpur. Fruits & Vegetables market The growers of fruits and vegetables in India mostly harvest their produce manually, transporting to orchards for grading & packing. Afterwards, the fruits and vegetable are shipped to wholesale markets by road. India being world’s second largest fruits producer with total of 44 mn MT (approx 10% of world’s production) and one of the largest vegetable producers in the world with around 86 mn MT, the new Sabji Mandi at Azadpur is Asia’s and one of the world’s biggest fruits and vegetable market in volume of produce handled contributed to the huge demand for cold storage facilities. Commission agents At the arrival of fruits at Azadpur, the commission agents take charge of the produce by offloading, storage and sale at a rate of 6% of the sale amount. During this process all the expenses involved are borne by the growers. As a way to get business, some of the commission agents offer growers advance loans at the commencement of the fruits season and deduct the same amount when the produce is sold in the market. As the fruits reach the market, some will be sold on the same day while roughly 45% – 60% need cold storage facility to be sold at a later date or at the off-season. Wholesalers Wholesalers buy the produce from commission agents which in turn they sell to sub-wholesalers. Cold storage Industry A conventional cold storage facility can preserve fruits for a period of 10 days to 2 months depending of the type of the fruits while a CA or GC storage facility can double or triple this period respectively. The total capacity of storage facilities available was nearly 10.35 mn MT against total produce of over 130 mn MT, this can demonstrate the very high demand of storage facilities. IPSCL has differentiated itself from the market by upgrading the facility to state-of-the-art Gas Controlled cold storage (3900 MT, around 78% of the capacity), Controlled Atmosphere Cold Storage (100 MT, 2% of the available capacity) and a Deep Freezer storage (1000 MT, 20% of the capacity). Besides, there was a lack of refrigerated trucks to mobilize the produce from the growers’ location to the wholesale markets and from the markets to sub-wholesalers or retailers. The refrigerated trucks play a vital role in ensuring the freshness, quality and firmness of the produce. Government Aimed at encouraging the growing industry, the GoI offered a capital investment Subsidy Scheme for the construction, expansion and / or modification of cold storage units. Competition Besides IPCSL, no other cold storage facility is operating under the GC or GA technology. However due to this technology differentiation, IPCSL was operating at a very high cost in comparison to the other facilities. In fact, the conventional cold storage facilities where offering their clients a price almost equal to IPCSL’s operating cost. Customers IPCSL has two types of customer, commission agents & wholesalers who store their fruits to sell at a later date or off-season. The fruits and vegetables market has seen about 60% growth owing to the increase in income of the middle-class category in India. Observations The cold storage services is a price driven over quality driven market, the cold storage service providers currently in business are not having any points of differentiation which led to a price war and very slim margins. The very low profit margins have restricted the competitors from changing out their old technology storage facilities to an international standard facility. Some of the customers (wholesalers & commission agents) are not highly appreciating the cold storage services especially during the winter season where the fruits and vegetables don’t get affected much due to the very low temperature. In the case of conventional cold storage facility, the only way to make decent profit is by maximizing the sales volume and effectively utilizing capacity available (dynamic facility management system). This would give some cost preference over competitors allowing to capture bigger market share and make higher profit margin. In the case of IPCSL, the GC & CA storage technology is giving them a market edge to charge a premium price. However the customers should value the added services provided over the difference in charges. Other than the limitation in the period fruits and vegetables can be stored in the conventional cold storage facility, there are additional quantities wasted during storage & handling which will not be the case in GC or CA operated facility. This will decrease the cost per kg and increase the wholesalers / commission agent profit margin. IPCSL didn’t focus much on marketing of their new state-of-the-art cold storage facility; instead, they focused on the value they add to the fruits and vegetables industry. As rightly quoted by one of the company’s senior managers to Sanjay, the fruits and markets industry lack formal education. It takes time to show value and build confidence. However, to speed up the learning curve and attract customers, price & marketing strategy can play a crucial factor especially in the introductory stage. Recommendations & Suggestions Marketing: IPCSL should offer the large, large-middle or all clients a free, discounted or at-cost trial to demonstrate the difference in quality between the conventional facilities against the GC & CA operated ones. IPCSL should organize visits / tours for the large wholesalers to the facility to show how differently can they store the fruits and vegetables and exhibit the benefits to the prospective clients. As IPCSL is a high-end cold storage service provider, the marketing strategy should focus on the clients of the same level i.e. the wholesalers and commission agents dealing with organized retail stores, hotels etc. these clients are willing to pay some extra charges for higher quality fruits and vegetables which cannot be matched by any other cold storage facility in the market. Price: IPCSL should strategize a price discrimination based on the volume similar to what the competitors are doing. The price discrimination will attract the large exporters, high volume wholesalers and commission agents towards IPCSL. From IPCSL’s revenue statement; it is obvious that more than INR 6 mn is fixed cost while variable cost is around INR 7.5 per 20kg box. IPCSL priced their services at INR 60 for imported fruits and INR 20 for Indian fruits against INR 10 per 20kg box by competitors for imported and Indian fruits. Alternatively, IPCSL should price their services at the point where they maximally utilize their 5000 MT facility. The determined price along with the 5000 MT sales volume (if achieved) should cover up for the variable cost (INR 7.5 per 20kg box) as well as the very high fixed cost.

The Twilight Saga 2: New Moon Chapter 21 VERDICT

WE WERE IN A BRIGHTLY LIT, UNREMARKABLE HALLWAY. The walls were off-white, the floor carpeted in industrial gray. Common rectangular fluorescent lights were spaced evenly along the ceiling. It was warmer here, for which I was grateful. This hall seemed very benign after the gloom of the ghoulish stone sewers. Edward didn't seem to agree with my assessment. He glowered darkly down the long hallway, toward the slight, black shrouded figure at the end, standing by an elevator. He pulled me along, and Alice walked on my other side. The heavy door creaked shut behind us, and then there was the thud of a bolt sliding home. Jane waited by the elevator, one hand holding the doors open for us. Her expression was apathetic. Once inside the elevator, the three vampires that belonged to the Volturi relaxed further. They threw back their cloaks, letting the hoods fall back on their shoulders. Felix and Demetri were both of a slightly olive complexionit looked odd combined with their chalky pallor. Felix's black hair was cropped short, but Demetri's waved to his shoulders. Their irises were deep crimson around the edges, darkening until they were black around the pupil. Under the shrouds, their clothes were modern, pale, and nondescript. I cowered in the corner, cringing against Edward. His hand still rubbed against my arm. He never took his eyes off Jane. The elevator ride was short; we stepped out into what looked like a posh office reception area. The walls were paneled in wood, the floors carpeted in thick, deep green. There were no windows, but large, brightly lit paintings of the Tuscan countryside hung everywhere as replacements. Pale leather couches were arranged in cozy groupings, and the glossy tables held crystal vases full of vibrantly colored bouquets. The flowers' smell reminded me of a funeral home. In the middle of the room was a high, polished mahogany counter. I gawked in astonishment at the woman behind it. She was tall, with dark skin and green eyes. She would have been very pretty in any other companybut not here. Because she was every bit as human as I was. I couldn't comprehend what this human woman was doing here, totally at ease, surrounded by vampnes. She smiled politely in welcome. â€Å"Good afternoon, Jane,† she said. There was no surprise in her face as she glanced at Jane's company. Not Edward, his bare chest glinting dimly in the white lights, or even me, disheveled and comparatively hideous. Jane nodded. â€Å"Gianna.† She continued toward a set of double doors in the back of the room, and we followed. As Felix passed the desk, he winked at Gianna, and she giggled. On the other side of the wooden doors was a different kind of reception. The pale boy in the pearl gray suit could have been Jane's twin. His hair was darker, and his lips were not as full, but he was just as lovely. He came forward to meet us. He smiled, reaching for her. â€Å"Jane.† â€Å"Alec,† she responded, embracing the boy. They kissed each other's cheeks on both sides. Then he looked at us. â€Å"They send you out for one and you come back with two and a half,† he noted, looking at me. â€Å"Nice work.† She laughedthe sound sparkled with delight like a baby's cooing. â€Å"Welcome back, Edward,† Alec greeted him. â€Å"You seem in a better mood.† â€Å"Marginally,† Edward agreed in a flat voice. I glanced at Edward's hard face, and wondered how his mood could have been darker before. Alec chuckled, and examined me as I clung to Edward's side. â€Å"And this is the cause of all the trouble?† he asked, skeptical. Edward only smiled, his expression contemptuous. Then he froze. â€Å"Dibs,† Felix called casually from behind. Edward turned, a low snarl building deep in his chest. Felix smiledhis hand was raised, palm up; he curled his fingers twice, inviting Edward forward. Alice touched Edward's arm. â€Å"Patience,† she cautioned him. They exchanged a long glance, and I wished I could hear what she was telling him. I figured that it was something to do with not attacking Felix, because Edward took a deep breath and turned back to Alec. â€Å"Aro will be so pleased to see you again,† Alec said, as if nothing had passed. â€Å"Let's not keep him waiting,† Jane suggested. Edward nodded once. Alec and Jane, holding hands, led the way down yet another wide, ornate hallwould there ever be an end? They ignored the doors at the end of the halldoors entirely sheathed in goldstopping halfway down the hall and sliding aside a piece of the paneling to expose a plain wooden door. It wasn't locked. Alec held it open for Jane. I wanted to groan when Edward pulled me through to the other side of the door. It was the same ancient stone as the square, the alley, and the sewers. And it was dark and cold again. The stone antechamber was not large. It opened quickly into a brighter, cavernous room, perfectly round like a huge castle turret which was probably exactly what it was. Two stories up, long window slits threw thin rectangles of bright sunlight onto the stone floor below. There were no artificial lights. The only furniture in the room were several massive wooden chairs, like thrones, that were spaced unevenly, flush with the curving stone walls. In the very center of the circle, in a slight depression, was another drain. I wondered if they used it as an exit, like the hole in the street. The room was not empty. A handful of people were convened in seemingly relaxed conversation. The murmur of low, smooth voices was a gentle hum in the air. As I watched, a pair of pale women in summer dresses paused in a patch of light, and, like prisms, their skin threw the light in rainbow sparkles against the sienna walls. The exquisite faces all turned toward our party as we entered the room. Most of the immortals were dressed in inconspicuous pants and shirtsthings that wouldn't stick out at all on the streets below. But the man who spoke first wore one of the long robes. It was pitch-black, and brushed against the floor. For a moment, I thought his long, jet-black hair was the hood of his cloak. â€Å"Jane, dear one, you've returned!† he cried in evident delight. His voice was just a soft sighing. He drifted forward, and the movement flowed with such surreal grace that I gawked, my mouth hangmg open. Even Alice, whose every motion looked like dancing, could not compare. I was only more astonished as he floated closer and I could see his face. It was not like the unnaturally attractive faces that surrounded him (for he did not approach us alone; the entire group converged around him, some following, and some walking ahead of him with the alert manner of bodyguards). I couldn't decide if his face was beautiful or not. I suppose the features were perfect. But he was as different from the vampires beside him as they were from me. His skin was translucently white, like onionskin, and it looked just as delicateit stood in shocking contrast to the long black hair that framed his face. I felt a strange, horrifying urge to touch his cheek, to see if it was softer than Edward's or Alice's, or if it was powdery, like chalk. His eyes were red, the same as the others around him, but the color was clouded, milky; I wondered if his vision was affected by the haze. He glided to Jane, took her face in his papery hands, kissed her lightly on her full lips, and then floated back a step. â€Å"Yes, Master.† Jane smiled; the expression made her look like an angelic child. â€Å"I brought him back alive, just as you wished.† â€Å"Ah, Jane.† He smiled, too. â€Å"You are such a comfort to me.† He turned his misty eyes toward us, and the smile brightenedbecame ecstatic. â€Å"And Alice and Bella, too!† he rejoiced, clapping his thin hands together. â€Å"This is a happy surprise! Wonderful!† I stared in shock as he called our names informally, as if we were old friends dropping in for an unexpected visit. He turned to our hulking escort. â€Å"Felix, be a dear and tell my brothers about our company. I'm sure they wouldn't want to miss this.† â€Å"Yes, Master.† Felix nodded and disappeared back the way we had come. â€Å"You see, Edward?† The strange vampire turned and smiled at Edward like a fond but scolding grandfather. â€Å"What did I tell you? Aren't you glad that I didn't give you what you wanted yesterday?† â€Å"Yes, Aro, I am,† he agreed, tightening his arm around my waist. â€Å"I love a happy ending.† Aro sighed. â€Å"They are so rare. But I want the whole story. How did this happen? Alice?† He turned to gaze at Alice with curious, misty eyes. â€Å"Your brother seemed to think you infallible, but apparently there was some mistake.† â€Å"Oh, I'm far from infallible.† She flashed a dazzling smile. She looked perfectly at ease, except that her hands were balled into tight little fists. â€Å"As you can see today, I cause problems as often as I cure them.† â€Å"You're too modest,† Aro chided. â€Å"I've seen some of your more amazing exploits, and I must admit I've never observed anything like your talent. Wonderful!† Alice flickered a glance at Edward. Aro did not miss it. â€Å"I'm sorry, we haven't been introduced properly at all, have we? It's just that I feel like I know you already, and I tend get ahead of myself. Your brother introduced us yesterday, in a peculiar way. You see, I share some of your brother's talent, only I am limited in a way that he is not.† Aro shook his head; his tone was envious. â€Å"And also exponentially more powerful,† Edward added dryly. He looked at Alice as he swiftly explained. â€Å"Aro needs physical contact to hear your thoughts, but he hears much more than I do. You know I can only hear what's passing through your head in the moment. Aro hears every thought your mind has ever had.† Alice raised her delicate eyebrows, and Edward inclined his head. Aro didn't miss that either. â€Å"But to be able to hear from a distance† Aro sighed, gesturing toward the two of them, and the exchange that had just taken place. â€Å"That would be so convenient.† Aro looked over our shoulders. All the other heads turned in the same direction, including Jane, Alec, and Demetri, who stood silently beside us. I was the slowest to turn. Felix was back, and behind him floated two more black-robed men. Both looked very much like Aro, one even had the same flowing black hair. The other had a shock of snow-white hairthe same shade as his facethat brushed against his shoulders. Their faces had identical, paper-thin skin. The trio from Carlisle's painting was complete, unchanged by the last three hundred years since it was painted. â€Å"Marcus, Caius, look!† Aro crooned. â€Å"Bella is alive after all, and Alice is here with her! Isn't that wonderful?† Neither of the other two looked as if wonderful would be their first choice of words. The dark-haired man seemed utterly bored, like he'd seen too many millennia of Aro's enthusiasm. The other's hice was sour under the snowy hair. Their lack of interest did not curb Aro's enjoyment. â€Å"Let us have the story,† Aro almost sang in his feathery voice. The white-haired ancient vampire drifted away, gliding toward one of the wooden thrones. The other paused beside Aro, and he reached his hand out, at first I thought to take Aro's hand. But he just touched Aro's palm briefly and then dropped his hand to his side. Aro raised one black brow. I wondered how his papery skin did not crumple in the effort. Edward snorted very quietly, and Alice looked at him, curious. â€Å"Thank you, Marcus,† Aro said. â€Å"That's quite interesting.† I realized, a second late, that Marcus was letting Aro know his thoughts. Marcus didn't look interested. He glided away from Aro to join the one who must be Caius, seated against the wall. Two of the attending vampires followed silently behind himbodyguards, like I'd thought before. I could see that the two women in the sundresses had gone to stand beside Caius in the same manner. The idea of any vampire needing a guard was faintly ridiculous to me, but maybe the ancient ones were as frail as their skin suggested. Aro was shaking his head. â€Å"Amazing,†Ã¢â‚¬Ëœ he said. â€Å"Absolutely amazing.† Alice's expression was frustrated. Edward turned to her and explained again in a swift, low voice. â€Å"Marcus sees relationships. He's surprised by the intensity of ours.† Aro smiled. â€Å"So convenient,† he repeated to himself. Then he spoke to us. â€Å"It takes quite a bit to surprise Marcus, I can assure you.† I looked at Marcus's dead face, and I believed that. â€Å"It's just so difficult to understand, even now,† Aro mused, staring at Edward's arm wrapped around me. It was hard for me to follow Aro's chaotic train of thought. I struggled to keep up. â€Å"How can you stand so close to het like that?† â€Å"It's not without effort,† Edward answered calmly. â€Å"Butstillla tua cantante! What a waste!† Edward chuckled once without humor. â€Å"I look at it more as a price.† Aro was skeptical. â€Å"A very high price.† â€Å"Opportunity cost.† Aro laughed. â€Å"If I hadn't smelled her through your memories, I wouldn't have believed the call of anyone's blood could be so strong. I've never felt anything like it myself. Most of us would trade much for such a gift, and yet you† â€Å"Waste it,† Edward finished, his voice sarcastic now. Aro laughed again. â€Å"Ah, how I miss my friend Carlisle! You remind me of himonly he was not so angry.† â€Å"Carlisle outshines me in many other ways as well.† â€Å"I certainly never thought to see Carlisle bested for self-control of all things, but you put him to shame.† â€Å"Hardly.† Edward sounded impatient. As if he were tired of the preliminaries. It made me more afraid; I couldn't help but try to imagine what he expected would follow. â€Å"I am gratified by his success,† Aro mused. â€Å"Your memories of him are quite a gift for me, though they astonish me exceedingly. I am surprised by how it pleases me, his success in this unorthodox path he's chosen. I expected that he would waste, weaken with time. I'd scoffed at his plan to find others who would share his peculiar vision. Yet, somehow, I'm happy to be wrong.† Edward didn't reply. â€Å"But your restraint!† Aro sighed. â€Å"I did not know such strength was possible. To inure yourself against such a siren call, not just once but again and againif I had not felt it myself, I would not have believed.† Edward gazed back at Aro's admiration with no expression. I knew his face well enoughtime had not changed thatto guess at something seething beneath the surface. I fought to keep my breathing even. â€Å"Just remembering how she appeals to you† Aro chuckled. â€Å"It makes me thirsty.† Edward tensed. â€Å"Don't be disturbed,† Aro reassured him. â€Å"I mean her no harm. But I am so curious, about one thing in particular.† He eyed me with bright interest. â€Å"May I?† he asked eagerly, lifting one hand. â€Å"Ask her,† Edward suggested in a flat voice. â€Å"Of course, how rude of me!† Aro exclaimed. â€Å"Bella,† he addressed me directly now. â€Å"I'm fascinated that you are the one exception to Edward's impressive talentso very interesting that such a thing should occur! And I was wondering, since our talents are similar in many ways, if you would be so kind as to allow me to tryto see if you are an exception for me, as well?† My eyes flashed up to Edward's face in terror. Despite Aro's overt politeness, I didn't believe I really had a choice. I was horrified at the thought of allowing him to touch me, and yet also perversely intrigued by the chance to feel his strange skin. Edward nodded in encouragementwhether because he was sure Aro would not hurt me, or because there was no choice, I couldn't tell. I turned back to Aro and raised my hand slowly in front of me. It was trembling. He glided closer, and I believe he meant his expression to be reassuring. But his papery features were too strange, too alien and frightening, to reassure. The look on his face was more confident than his words had been. Aro reached out, as if to shake my hand, and pressed his insubstantial-looking skin against mine. It was hard, but felt brittleshale rather than graniteand even colder than I expected. His filmy eyes smiled down at mine, and it was impossible to look away. They were mesmerizing in an odd, unpleasant way. Aro's face altered as I watched. The confidence wavered and became first doubt, then incredulity before he calmed it into a friendly mask. â€Å"So very interesting,† he said as he released my hand and drifted back. My eyes flickered to Edward, and, though his face was composed, I thought he seemed a little smug. Aro continued to drift wnh a thoughtful expression. He was quiet for a moment, his eyes flickering between the three of us. Then, abruptly, he shook his head. â€Å"A first,† he said to himself â€Å"I wonder if she is immune to our other talents Jane, dear?† â€Å"No!† Edward snarled the word. Alice grabbed his arm with a restraining hand. He shook her off. Little Jane smiled up happily at Aro. â€Å"Yes, Master?† Edward was truly snarling now, the sound ripping and tearing from him, glaring at Aro with baleful eyes. The room had gone still, everyone watching him with amazed disbelief, as if he were committing some embarrassing social faux pas. I saw Felix grin hopefully and move a step forward. Aro glanced at him once, and he froze in place, his grin turning to a sulky expression. Then he spoke to Jane. â€Å"I was wondering, my dear one, if Bella is immune to you.† I could barely hear Aro over Edward's furious growls. He let go of me, moving to hide me from their view. Caius ghosted in our direction, with his entourage, to watch. Jane turned toward us with a beatific smile. â€Å"Don't!† Alice cried as Edward launched himself at the little girl. Before I could react, before anyone could jump between them, before Aro's bodyguards could tense, Edward was on the ground. No one had touched him, but he was on the stone floor writhing in obvious agony, while I stared in horror. Jane was smiling only at him now, and it all clicked together. What Alice had said about formidable gifts , why everyone treated Jane with such deference, and why Edward had thrown himself in her path before she could do that to me. â€Å"Stop!† I shrieked, my voice echoing in the silence, jumping forward to put myself between them. But Alice threw her arms around me in an unbreakable grasp and ignored my struggles. No sound escaped Edward's lips as he cringed against the stones. It felt like my head would explode from the pain of watching this. â€Å"Jane,† Aro recalled her in a tranquil voice. She looked up quickly, still smiling with pleasure, her eyes questioning. As soon as Jane looked away, Edward was still. Aro inclined his head toward me. Jane turned her smile in my direction. I didn't even meet her gaze. I watched Edward from the prison of Alice's arms, still struggling pointlessly. â€Å"He's fine,† Alice whispered in a tight voice. As she spoke, he sat up, and then sprang lightly to his feet. His eyes met mine, and they were horror-struck. At first I thought the horror was for what he had just suffered. But then he looked quickly at Jane, and back to meand his face relaxed into relief. I looked at Jane, too, and she no longer smiled. She glared at me, her jaw clenched with the intensity of her focus. I shrank back, waiting for the pain. Nothing happened. Edward was by my side again. He touched Alice's arm, and she surrendered me to him. Aro started to laugh. â€Å"Ha, ha. ha,† he chuckled. â€Å"This is wonderful!† Jane hissed in frustration, leaning forward like she was preparing to spring. â€Å"Don't be put out, dear one,† Aro said in a comforting tone, placing a powder-light hand on her shoulder. â€Å"She confounds us all.† Jane's upper lip curled back ever her teeth as she continued to glare at me. â€Å"Ha, ha, ha,† Aro chortled again. â€Å"You're very brave, Edward, to endure in silence. I asked Jane to do that to me oncejust out of curiosity.† He shook his head in admiration. Edward glared, disgusted. â€Å"So what do we do with you now?† Aro sighed. Edward and Alice stiffened. This was the part they'd been waiting for. I began to tremble. â€Å"I don't suppose there's any chance that you've changed your mind?† Aro asked Edward hopefully. â€Å"Your talent would be an excellent addition to our little company.† Edward hesitated. From the corner of my eye, I saw both Felix and Jane grimace. Edward seemed to weigh each word before he spoke it. â€Å"I'd rather not.† â€Å"Alice?† Aro asked, still hopeful. â€Å"Would you perhaps be interested in joining with us?† â€Å"No, thank you,† Alice said. â€Å"And you, Bella?† Aro raised his eyebrows. Edward hissed, low in my ears. I stared at Aro blankly. Was he joking? Or was he really asking me if I wanted to stay for dinner? It was the white-haired Caius who broke the silence. â€Å"What?† he demanded of Aro; his voice, though no more than a whisper, was flat. â€Å"Caius, surely you see the potential,† Aro chided him affectionately. â€Å"I haven't seen a prospective talent so promising since we found Jane and Alec. Can you imagine the possibilities when she is one of us?† Caius looked away with a caustic expression. Jane's eyes sparked with indignation at the comparison. Edward fumed beside me. I could hear a rumble in his chest, building toward a growl. I couldn't let his temper get him hurt. â€Å"No, thank you,† I spoke up in barely more than a whisper, my voice breaking in fright. Aro sighed. â€Å"That's unfortunate. Such a waste.† Edward hissed. â€Å"Join or die, is that it? I suspected as much when we were brought to this room. So much for your laws.† The tone of his voice surprised me. He sounded irate, but there was something deliberate about his deliveryas if he'd chosen his words with great care. â€Å"Of course not.† Aro blinked, astonished. â€Å"We were already convened here, Edward, awaiting Heidi's return. Not for you.† â€Å"Aro,† Caius hissed. â€Å"The law claims them.† Edward glared at Caius. â€Å"How so?† he demanded. He must have known what Caius was thinking, but he seemed determined to make him speak it aloud. Caius pointed a skeletal finger at me. â€Å"She knows too much. You have exposed our secrets.† His voice was papery thin, just like his skin. â€Å"There are a few humans in on your charade here, as well,† Edward reminded him, and I thought of the pretty receptionist below. Caius's face twisted into a new expression. Was it supposed to be a smiled. â€Å"Yes,† he agreed. â€Å"But when they are no longer useful to us, they will serve to sustain us. That is not your plan for this one. If she betrays our secrets, are you prepared to destroy her? I think not,† he scoffed. â€Å"I wouldn't,† I began, still whispering. Caius silenced me with an icy look. â€Å"Nor do you intend to make her one of us,† Caius continued. â€Å"Therefore, she is a vulnerability. Though it is true, for this, only her life is forfeit. You may leave if you wish.† Edward bared his teeth. â€Å"That's what I thought,† Caius said, with something akin to pleasure. Felix leaned forward, eager. â€Å"Unless† Aro interrupted. He looked unhappy with the way the conversation had gone. â€Å"Unless you do intend to give her immortality?† Edward pursed his lips, hesitating for a moment before he answered. â€Å"And if I do?† Aro smiled, happy again. â€Å"Why, then you would be free to go home and give my regards to my friend Carlisle.† His expression turned more hesitant. â€Å"But I'm afraid you would have to mean it.† Aro raised his hand in front of him. Caius, who had begun to scowl furiously, relaxed. Edward's lips tightened into a fierce line. He stared into my eyes, and I stared back. â€Å"Mean it,† I whispered. â€Å"Please.† Was it really such a loathsome idea? Would he rather die than change me? I felt like I'd been kicked in the stomach. Edward stared down at me with a tortured expression. And then Alice stepped away from us, forward toward Aro. We turned to watch her. Her hand was raised like his. She didn't say anything, and Aro waved off his anxious guard as they moved to block her approach. Aro met her halfway, and took her hand with an eager, acquisitive glint in his eyes. He bent his head over their touching hands, his eyes closing as he concentrated. Alice was motionless, her face blank. I heard Edward's teeth snap together. No one moved. Aro seemed frozen over Alice's hand. The seconds passed and I grew more and more stressed, wondering how much time would pass before it was too much time. Before it meant something was wrongmore wrong than it already was. Another agonizing moment passed, and then Aro's voice broke the silence. â€Å"Ha, ha, ha,† he laughed, his head still bent forward. He looked up slowly, his eyes bright with excitement. â€Å"That was fascinating!† Alice smiled dryly. â€Å"I'm glad you enjoyed it.† â€Å"To see the things you've seenespecially the ones that haven't happened yet!† He shook his head in wonder. â€Å"But that will,† she reminded him, voice calm. â€Å"Yes, yes, it's quite determined. Certainly there's no problem.† Caius looked bitterly disappointeda feeling he seemed to share with Felix and Jane. â€Å"Aro,† Caius complained. â€Å"Dear Caius,† Aro smiled. â€Å"Do not fret. Think of the possibilities! They do not join us today, but we can always hope for the future. Imagine the joy young Alice alone would bring to our little household Besides, I'm so terribly curious to see how Bella turns out!† Aro seemed convinced. Did he not realize how subjective Alice's visions were.' That she could make up her mind to transform me today, and then change it tomorrow? A million tiny decisions, her decisions and so many others', tooEdward'scould alter her path, and with that, the future. And would it really matter that Alice was willing, would it make any difference if I did become a vampire, when the idea was so repulsive to Edward? If death was, to him, a better alternative than having me around forever, an immortal annoyance? Terrified as I was, I felt myself sinking down into depression, drowning in it â€Å"Then we are free to go now?† Edward asked in an even voice. â€Å"Yes, yes,† Aro said pleasantly. â€Å"But please visit again. It's been absolutely enthralling!† â€Å"And we will visit you as well,† Caius promised, his eyes suddenly half-closed like the heavy-lidded gaze of a lizard. â€Å"To be sure that you follow through on your side. Were I you, I would not delay too long. We do not offer second chances.† Edward's jaw clenched tight, but he nodded once. Caius smirked and drifted back to where Marcus still sat, unmoving and uninterested. Felix groaned. â€Å"Ah, Felix.† Aro smiled, amused. â€Å"Heidi will be here at any moment. Patience.† â€Å"Hmm.† Edward's voice had a new edge to it. â€Å"In that case, perhaps we'd better leave sooner rather than later.† â€Å"Yes,† Aro agreed. â€Å"That's a good idea. Accidents do happen. Please wait below until after dark, though, if you don't mind.† â€Å"Of course,† Edward agreed, while I cringed at the thought of waiting out the day before we could escape. â€Å"And here,† Aro added, motioning to Felix with one finger. Felix came forward at once, and Aro unfastened the gray cloak the huge vampire wore, pulling from his shoulders. He tossed it to Edward. â€Å"Take this. You're a little conspicuous.† Edward put the long cloak on, leaving the hood down. Aro sighed. â€Å"It suits you.† Edward chuckled, but broke off suddenly, glancing over his shoulder. â€Å"Thank you, Aro. We'll wait below.† â€Å"Goodbye, young friends,† Aro said, his eyes bright as he stared in the same direction. â€Å"Let's go,† Edward said, urgent now. Demetri gestured that we should follow, and then set off the way we'd come in, the only exit by the look of things. Edward pulled me swiftly along beside him. Alice was close by my other side, her face hard. â€Å"Not fast enough,† she muttered. I stared up at her, frightened, but she only seemed chagrined. It was then that I first heard the babble of voicesloud, rough voicescoming from the antechamber. â€Å"Well this is unusual,† a man's coarse voice boomed. â€Å"So medieval,† an unpleasantly shrill, female voice gushed back. A large crowd was coming through the little door, filling the smaller stone chamber. Demetri motioned for us to make room. We pressed back against the cold wall to let them pass. The couple in front, Americans from the sound of them, glanced around themselves with appraising eyes. â€Å"Welcome, guests! Welcome to Volterra!† I could hear Aro sing from the big turret room. The rest of them, maybe forty or more, filed in after the couple. Some studied the setting like tourists. A few even snapped pictures. Others looked confused, as if the story that had led them to this room was not making sense anymore. I noticed one small, dark woman in particular. Around her neck was a rosary, and she gripped the cross tightly in one hand. She walked more slowly than the others, touching someone now and then and asking a question in an unfamiliar language. No one seemed to understand her, and her voice grew more panicked. Edward pulled my face against his chest, but it was too late. I already understood. As soon as the smallest break appeared, Edward pushed me quickly toward the door. I could feel the horrified expression on my face, and the tears beginning to pool in my eyes. The ornate golden hallway was quiet, empty except for one gorgeous, statuesque woman. She stared at us curiously, me in particular. â€Å"Welcome home, Heidi,† Demetri greeted her from behind us. Heidi smiled absently. She reminded me of Rosalie, though they looked nothing alikeit was just that her beauty, too, was exceptional, unforgettable. I couldn't seem to look away. She was dressed to emphasize that beauty. Her amazingly long legs, darkened with tights, were exposed by the shortest of miniskirts. Her top was long-sleeved and high-necked, but extremely close-fitting, and constructed of red vinyl. Her long mahogany hair was lustrous, and her eyes were the strangest shade of violeta color that might result from blue-tinted contacts over red irises. â€Å"Demetri,† she responded in a silky voice, her eyes flickering between my face and Edward's gray cloak. â€Å"Nice fishing,† Demetri complimented her, and I suddenly understood the attention-grabbing outfit she wore she was not only the fisherman, but also the bait. â€Å"Thanks.† She flashed a stunning smile. â€Å"Aren't you coming?† â€Å"In a minute. Save a few for me.† Heidi nodded and ducked through the door with one last curious look at me. Edward set a pace that had me running to keep up. But we still couldn't get through the ornate door at the end of the hallway before the screaming started.

Beowulf And The Germanic Heroic Code Of Values,...

Every culture has its own set of values, beliefs, and customs. These values, beliefs and customs create each unique culture, and these cultural constructions are directly and indirectly acquired throughout the lifetime of the culture. A major part of culture is literature; the literature of the time reflects upon daily life and society during the time that the story was composed. Beowulf, an epic poem, is one of the most important and only works of Anglo-Saxon literature. Much like a fable, the epic poem of Beowulf had lessons that taught the people within this society how to behave. This essay will outline how the importance of Christianity and the Germanic Heroic Code in Beowulf are revealed throughout the epic poem as important aspects of the Anglo-Saxon culture as a whole. The Germanic warrior society had an unspoken code – a list of rules that warriors and kings alike must uphold. The main aspects of this code for warriors were strength, courage, loyalty and honour; and for kings were loyalty to his warriors, generosity and a sense of responsibility. The character of Beowulf represents both sides of this Code very well. Hrothgar, King of Denmark, represents the nobility’s side of the code: Then Halfdane’s son presented Beowulf with a gold standard as a victory gift†¦ that was both precious object and token of honor†¦ And so their leader, the lord and guard of coffer and strongroom, with customary grace bestowed upon Beowulf both sets of gifts. A fairShow MoreRelatedAnglo-Saxon Heroic Poetry5673 Words   |  23 Pagesthe Heroic, the sources of which are pre-Christian Germanic myth, history and custom; and the Christian. Heroic, or Epic Poetry belongs to one of these two types and refers to long narrative poems celebrating the great deeds of one or more legendary heroes, in a grand, ceremonious style. In its strict use by literary critics, the terms Heroic Poetry or Epic are applied to a work that meets the following criteria: such a poem must be related in an elevated style, and centered upon a heroic or quasi-divineRead MoreEssay on Beowulf is a Christian Poem1371 Words   |  6 Pages The epic poem Beowulf, was written sometime in the eighth century by an unknown author. It was based on legend passed down over time. Prior to the time the poem was written, Anglo Saxons had converted from Germanic Paganism to Christianity. Some people argue that it was a Pagan poem rewritten by a person or persons educated in Christianity. â€Å" has come down from heathen times and acquired its Christian character gradually and piecemeal from a succession of minstrels.† ( Hector Monro ChadwickRead MoreTrace the Development of English Lit During Any One Period...as Part of Your Discussion Highlight How Significant Events in the Influence the Writing...Additionally Show How Characteristics of the Genre the Writer Uses3085 Words   |  13 PagesAnglo-Saxon. These are: Angles from Angel in South-West Denmark, Saxon from North-West Germany, and Jute from Jutland in Central Denmark. These three main ethnic groups have made up most of the Anglo-Saxon society. However; smaller group of people from Germanic ethnic group were also associated with the Anglo-Saxons. These people shared the same language but were each ruled by different strong warriors who invaded and conquered Britain while the Romans were still in control. The Angles and the Saxon tribeRead More The Anglo-Saxon poems, The Wanderer, The Seafarer, and The Wife’s Lament3469 Words   |  14 PagesWife’s Lament† The Old English, or Anglo-Saxon, era of England lasted from about 450-1066 A.D. The tribes from Germany that conquered Britain in the fifth century carried with them both the Old English language and a detailed poetic tradition. The tradition included alliteration, stressed and unstressed syllables, but more importantly, the poetry was usually mournful, reflecting on suffering and loss.1These sorrowful poems from the Anglo Saxon time period are mimetic to the Anglo-Saxons themselves;Read MoreAnalysis Of The Poem Beowulf 3700 Words   |  15 PagesAssignment Beowulf 1. This epic poem is one of the first works composed in the English language. It also tells us about the Anglo-Saxon people who lived in England and their culture. What values did these people support? What ideals did they try to live by? How do their values compare to our values today? Try to find similarities between our culture and this ancient culture. Use examples from text to support your points. The Anglo-Saxon people in England at this time followed the Germanic heroic codeRead More Comparing and Contrasting the Monsters of Beowulf with Today ´s Criminals3020 Words   |  13 Pagesstory about heroes and villains, the monsters involved are often characterized as the evil ones and, consequently, receive no justice under the law. Throughout the epic story Beowulf, the hero of the story encounters three monsters that are threats to society: Grendel, Grendel’s mother, and the dragon. The monsters in Beowulf are quickly targeted and destroyed because of the harm they cause to society. However, upon further examination of the monsters and the motives for their actions, the readerRead MoreLiterature Reflecting Religious and Political Ideals3433 Words   |  14 PagesEach of these four texts represents a distinct period of history, with Beowulf depicting the Norse and Anglo-Saxon heroes of the Early Middle Ages (500-1000 AD), before the Norman Conquest of Britain in 1066. In this era, England and northern Europe were in the process of becoming Christianized and Beowulf was originally the story of a pagan hero with supernatural powers who had been turned into a Christian knight and holy warrior by the time the story was written down in the 9th Century. His mission